Training Resources
To access the OACU-sponsored training courses for Principal Investigators, animal users, and ACUC members, please click here (external link)
The training tools and multimedia resources provided on this website are intended to assist investigators to learn proper technical procedure methodology. Additional training and experience will be necessary in order to become proficient in these techniques. If you are an NIH employee, and are unsure of where to obtain additional training, please contact OACU at TrainingOACU@mail.nih.gov (email) or 301-496-5424 for assistance.
- Working Safely with Nonhuman Primates (external link) (English)
- Working Safely with Nonhuman Primates (PDF file) (English Script)
- Medidas de Seguirdad cuiando se Trabaja con Primates (external link) (Spanish)
- Medidas de Seguirdad cuiando se Trabaja con Primates (PDF file) (Spanish Script)
Monkey B Virus Safety Training Documents
NIH Policy Manual 3044-2 requires that all personnel working directly with nonhuman primates or that regularly enter nonhuman primate facilities undergo safety awareness training prior to unsupervised work. The documents provided on this page are part of the resources used by the trainers at NIH to conduct the course “Working Safely with Nonhuman Primates.” The trainers have been qualified to conduct this safety awareness training within the NIH Intramural Animal Program. Any questions regarding these resources should be directed to the Office of Animal Care and Use.
Training Package components:
- Video/CD/DVD Quiz (PDF file)
- Monkey B Virus Fact Sheet (PDF file)
- PM 3044-2 – Protection of NIH Personnel who Work with NHPs (external link)
- PM 1340 – NIH Occupational Safety and Health Management (external link)
- Video/CD/DVD Script (PDF file)
- Nonhuman Primate and Other Research Animal Bite, Scratch, Splash, and Exposure (BSSE) Guidelines (PDF file), 10/27/2022 REVISED
- NIH OMS Animal Exposure Program (PDF file)
- Training Registration Form:
- Microsoft Excel: Training Registration Form (Excel spreadsheet)
- PDF Format: Training Registration Form (PDF file)
- 2003 Emerging Inf. Dis. Article (PDF file)
- 2002 Clinical Inf. Dis. article (PDF file)
- 1998 MMWR article (PDF file)
- Fish Guts – A multimedia Guide to the Art & Science of Fish Anatomy, Health, & Necropsy (external link)
- Zebrafish Husbandry Association – Husbandry Info (external link)
- Zebrafish International Resource Center (ZIRC) (external link)
- Fish Genotyping Tutorial (PDF file)
- Zebrafish Model Organism Database (ZFIN) (external link)
- Mouse Care & Husbandry (Jackson Labs) (external link)
- Mouse Pup Appearance by Age Poster (PDF file) (Jackson Labs)
- Mouse Reproduction Facts (PDF file)
- Mouse Nomenclature Guidelines (Jackson Labs) (external link)
- NHLBI Conditional Allele Mouse Planner (CAMP) (external link) – Computer program for designing viable breeding strategies.
- NIH ARAC Guidelines for the Establishment and Use of Mouse Breeding Groups (PDF file)
- Visual Guide to Mouse Estrous Cycle (Jackson Labs) (PDF file)
Commercial Animal Sources:
- Mandibular (external link)
- Retro-orbital (external link)
- Saphenous (external link)
- Tail Nick (external link)
- Tail Vein Puncture (external link)
- Intracardiac (external link)
- Rodent Blood Collection Tutorial (PDF file)
- Effects of Blood Collection Site on Data in Mice (PDF file)
- Methods of Blood Collection in the Mouse (Hoff) (PDF file)
- NIH ARAC Guidelines for Survival Bleeding of Mice and Rats
- Intradermal Injection (external link)
- Intramuscular Injection (external link)
- Intraperitoneal Injection (external link)
- Intravenous Injection (external link)
- Oral Gavage (external link)
- Subcutaneous Injection (external link)
- Injection Routes, Maximum Needle Sizes, & Volumes Table (PDF file)
- Rodent Administration Route Tutorial (PDF file)
- Newcastle University Administration of Substances (external link)
Mouse Workshop: Safe Handling and Techniques classes are three and a half hour sessions that provide investigators with an opportunity to receive training on techniques using live mice. Instructors for these small group sessions are certified laboratory animal technologists and provide a good opportunity for learning basic skills. No prior experience with mice is required prior to attending this class.
Techniques covered in the classes: Basic handling and restraint, ear tag, ear punch, IP injection, gavage, tail vein injection, mandibular blood collection, tail snip for genotyping, IC blood collection and euthanasia. Note that techniques covered may vary depending on the skill level of students.
Skill Level – Novice: This course is meant to introduce scientists and other animal users to basic rodent handling and techniques as a foundation for further practice of these skills. No prior experience with mice is required prior to attending this class.
Prerequisite Training: None. No prior experience with mice is required prior to attending this class.
Cost: There is no registration fee for this course.
Register for Mouse Workshop (external link)
Restrictions: Due to limited workshop size, attendance is limited. Classes are currently being offered at the NIH Bethesda, MD campus only. Further resources are available at the IC level and we are happy to help connect you with those resources. Please contact OACU or your IC’s research animal program office for further information.
- Alternatives in Animal Research – NIH Library Training Course
- Animal Model and Model Organism Information Resources – NIH Library Training Course (external link)
- Atlas of Laboratory Mouse Histology (external link)
- E-Mouse Atlas Project (external link)
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NHGRI Training in Basic Biomethodology for Laboratory Mice CD and the NHGRI Training in Basic Biomethodology for Laboratory Rats CD
The National Human Genome Research Institute Office of Laboratory Animal Medicine has developed training CDs in Basic Biomethodology for Laboratory Mice and the Basic Biomethodology for Laboratory Rats. The CDs illustrate the most common practices used in the NIH intramural research program and were developed to promote proficiency in performing common techniques in the these two common rodent species. The CDs consist of training modules, table of contents, lists of definitions, and help and reference sections. To obtain copies of the CDs send your name, research institution affiliation, full mailing address and email address to: rodent-cd@mail.nih.gov (email). Requests are limited to only one of each CD.
Please note: The guidelines, policies, and resource documents featured on this page are intended for the NIH Intramural Research Program and are not to be considered guidance from the Office of Laboratory Animal Welfare (OLAW) or any other oversight entity.
This page was last updated on Tuesday, October 15, 2024